AP BIOLOGY

Detailed explanation-1: -RFLP analysis can be used as a form of genetic testing to observe whether an individual carries a mutant gene for a disease that runs in his or her family.

Detailed explanation-2: -RFLP is a difference in the size of DNA restriction fragment (restriction map) between individuals. It can serve as a useful genetic marker for the analysis and mapping of a large genome. RFLP is based on the principle that small differences in the DNA sequence can alter restriction enzyme cutting patterns.

Detailed explanation-3: -The main difference between RAPD and RFLP is that RAPD is a type of PCR which amplifies random fragments of DNA in a large template by using short primers whereas, in RFLP, one or more restriction enzymes digest the DNA sample, producing restriction fragments then separated by gel electrophoresis.

Detailed explanation-4: -The distance between the cleavage sites of a certain restriction endonuclease differs between individuals. Hence, the length of the DNA fragments produced by a restriction endonuclease will differ across both individual organisms and species.