LABORATORY REVIEW
MOLECULAR BIOLOGY
|
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
|
|
|
orientation
|
|
|
lowers activation energy
|
|
|
induced fit
|
|
|
affinity
|
Detailed explanation-1: -Correct answer: GLUT1 and GLUT3 have the highest affinity for glucose. The Michaelis constant, is the concentration of the substrate required to reach . The lower the, the higher the affinity is for the substrate.
Detailed explanation-2: -As a substrate binds to the active site, the active site changes shape a little, grasping the substrate more tightly and preparing to catalyze the reaction. After the reaction takes place, the products are released from the active site and diffuse away.
Detailed explanation-3: -An enzyme’s Km describes the substrate concentration at which half the enzyme’s active sites are occupied by substrate. A high Km means a lot of substrate must be present to saturate the enzyme, meaning the enzyme has low affinity for the substrate.
Detailed explanation-4: -I: Km is indicative of the affinity of an enzyme for its substrate. II: A high Km indicates high affinity for substrate binding. III: Km is a measure of the substrate concentration required for effective catalysis to occur.