LABORATORY REVIEW
MOLECULAR BIOLOGY
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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DNA polymerase
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Helicase
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Primase
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RNA polymerase
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Detailed explanation-1: -During transcription, the DNA of a gene serves as a template for complementary base-pairing, and an enzyme called RNA polymerase II catalyzes the formation of a pre-mRNA molecule, which is then processed to form mature mRNA (Figure 1).
Detailed explanation-2: -During transcription, enzymes called RNA polymerases build RNA molecules that are complementary to a portion of one strand of the DNA double helix (Figure 3).
Detailed explanation-3: -Because RNA polymerase II is responsible for the synthesis of mRNA from protein-coding genes, it has been the focus of most studies of transcription in eukaryotes.
Detailed explanation-4: -mRNA is synthesized in the nucleus using the nucleotide sequence of DNA as a template. This process requires nucleotide triphosphates as substrates and is catalyzed by the enzyme RNA polymerase II.
Detailed explanation-5: -The process of transcription begins when an enzyme called RNA polymerase (RNA pol) attaches to the template DNA strand and begins to catalyze production of complementary RNA.