LABORATORY REVIEW
MOLECULAR BIOLOGY
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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restriction endonucleases
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DNA polymerase
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ligase
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ligase
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Detailed explanation-1: -Restriction enzymes are also called “molecular scissors” as they cleave DNA at or near specific recognition sequences known as restriction sites. These enzymes make one incision on each of the two strands of DNA and are also called restriction endonucleases.
Detailed explanation-2: -restriction enzyme, also called restriction endonuclease, a protein produced by bacteria that cleaves DNA at specific sites along the molecule. In the bacterial cell, restriction enzymes cleave foreign DNA, thus eliminating infecting organisms.
Detailed explanation-3: -Restriction endonuclease: An enzyme from bacteria that can recognize specific base sequences in DNA and cut (restrict) the DNA at that site (the restriction site). Also called a restriction enzyme.
Detailed explanation-4: -1. ‘Restriction enzyme’ and ‘restriction endonuclease’ should be regarded as synonymous and the abbreviation REase (or in some cases, R) is preferred. However, the abbreviation ENase, which has been used extensively, may also be used.
Detailed explanation-5: –EcoRI and smaI are the two examples of restriction enzymes.