LABORATORY REVIEW
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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it separates tRNA in the A site from the growing polypeptide
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it binds to the stop codon in the A site in place of a tRNA
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it releases the amino acid from its tRNA to allow the amino acid to form a polypeptide
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it releases the ribosome from the ER to allow polypeptides into the cytosol
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Detailed explanation-1: -What is the function of the release factor during translation in eukaryotes? 0 It binds to the stop codon in the A site in place of a tRNA It supplies source 0f energy for termination of translation 0 It releases the amino acid from its tRNA to allow the amino acid to form a peptide bond.
Detailed explanation-2: -It binds to the stop codon in the A site in place of a tRNA. It releases the amino acid from its tRNA to allow the amino acid to be added to the growing polypeptide.
Detailed explanation-3: -Recognition of a stop codon by class I release factors (RF) leads to peptidyl-tRNA hydrolysis and the release of the newly synthesized protein from the ribosome (2).
Detailed explanation-4: -Translation termination in eukaryotes occurs in response to a stop codon in the ribosomal A-site and requires two release factors (RFs), eRF1 and eRF3, which bind to the A-site as an eRF1/eRF3/GTP complex with eRF1 responsible for codon recognition.
Detailed explanation-5: -Stop codons are recognized by eukaryotic release factor-1 (eRF1). The GTPase eRF3 then binds the complex of eRF1 bound to the ribosome. Binding of eRF1 to the ribosome at the stop codon A site triggers the hydrolysis and release of the peptide chain from the tRNA in the P site.