LABORATORY REVIEW
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Which enzyme is used to join nicks in the DNA strand?
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Primase
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DNA polymerase
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DNA ligase
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Endonuclease
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Explanation:
Detailed explanation-1: -DNA Ligase joins DNA fragments. Q. DNA ligases are the paste enzymes which help in joining the broken DNA fragments.
Detailed explanation-2: -The repair is completed by DNA ligase, an enzyme that seals single-strand nicks in double-stranded DNA.
Detailed explanation-3: -So, the correct answer is ‘Ligase’.
Detailed explanation-4: -Ligase, an enzyme that uses ATP to form bonds, is used in recombinant DNA cloning to join restriction endonuclease fragments that have annealed. The ligase commonly used is T4 DNA ligase, which was first isolated from E. coli that were infected with the lytic bacteriophage T4.
Detailed explanation-5: -So the correct option is ‘Ligase’.
There is 1 question to complete.