AP BIOLOGY

LABORATORY REVIEW

MOLECULAR BIOLOGY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Which of the following is used to cut DNA from organisms?
A
Gel Electrophoresis
B
Restriction Enzymes
C
Karyotypes
D
Haplotypes
Explanation: 

Detailed explanation-1: -Restriction endonucleases are enzymes which scan the DNA molecule for a particular nucleotide sequence. These are called recognition sequences. Once the endonuclease finds this sequence it halts and cuts the strand. Thus the correct answer is option C.

Detailed explanation-2: -A restriction enzyme is a protein isolated from bacteria that cleaves DNA sequences at sequence-specific sites, producing DNA fragments with a known sequence at each end. The use of restriction enzymes is critical to certain laboratory methods, including recombinant DNA technology and genetic engineering.

Detailed explanation-3: -Restriction enzymes, found naturally in bacteria, can be used to cut DNA fragments at specific sequences, while another enzyme, DNA ligase, can attach or rejoin DNA fragments with complementary ends.

Detailed explanation-4: -Restriction enzymes, also called restriction endonucleases, recognize a specific sequence of nucleotides in double stranded DNA and cut the DNA at a specific location.

Detailed explanation-5: -In genetic engineering, restriction enzymes are used for cutting DNA fragments. A restriction enzyme (or restriction endonuclease) is an enzyme that cuts any DNA at or near specific recognition nucleotide sequences (known as restriction sites).

There is 1 question to complete.