LABORATORY REVIEW
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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filter milk over enzyme immobilized in mesh
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hydrolysis of lactase
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hydrolysis of lactose
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adding digestive enzyme to dairy products
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Detailed explanation-1: -Lactase catalyzes the hydrolysis of the glycosidic bond in the disaccharide lactose, releasing galactose and glucose (Figure 2)5.
Detailed explanation-2: -In lactose free products, we have split the lactose into glucose and galactose for you. At most dairies we use technologies to first pass the milk through filters that remove 40% of the lactose. Next, we remove the remaining lactose by adding the enzyme lactase that can separate the two molecules.
Detailed explanation-3: -The hydrolysis of lactose gives galactose and glucose. The galactose is converted to glucose by the action of an NAD-dependent enzyme called UDP-galactose-4-epimerase.
Detailed explanation-4: -Hydrolysis of lactose can be carried out by heating at low pH (acid hydrolysis) or by enzymatic catalysis with the enzyme (lactase or ..beta..-D-galactosidase) either free in solution or immobilized by one of the several enzyme immobilization methods which are abundant in the literature.