LABORATORY REVIEW
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Growth in agar without the ampicillin by bacteria with the plasmid
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Growth in ampicillin agar by bacteria with the plasmid
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Growth in ampicillin agar by bacteria without plasmid
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Growth in agar without the ampicillin by bacteria without the plasmid
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Detailed explanation-1: -Ampicillin resistance gene is the most commonly used selection marker. When the plasmid containing this marker is transformed, it gives cells the ability to thrive in media containing ampicillin. The non-transformed cells die due to the presence of antibiotic.
Detailed explanation-2: -The bla genes from phage DNA were transferred by electroporation to sensitive host bacteria, which became resistant to ampicillin.
Detailed explanation-3: -b-lactamase enzyme is the product of the bla gene (genes are typically lower case and italicized) If a plasmid contains the bla gene, it will confer resistance to ampicillin to the host E. coli.
Detailed explanation-4: -No. You cannot tell if the bacteria are ampicillin resistant just by looking at them. Both types of bacteria (those that are ampicillin resistant and those that are ampicillin sensitive) look similar when cultured-think about the colonies on the LB starter plate and the colonies on the +pGLO LB/amp plate.