AP BIOLOGY

Detailed explanation-1: -The genetically identical plants produced by plant tissue culture are collectively referred to as ‘somaclones’.

Detailed explanation-2: -The basic principle of single cell culture is the isolation of large number of intact living cells and cultures them on a suitable nutrient medium for their requisite growth and development. Single cells can be isolated from a variety of tissue and organ of green plant as well as from callus tissue and cell suspension.

Detailed explanation-3: -Plant regeneration is the major outcome of plant tissue culture, which is based on the principle of totipotency. Plant regeneration can be achieved by organogenesis and somatic embryogenesis (Fig. 6.1). Organogenesis means formation of organs from the cultured explants.

Detailed explanation-4: -Plant cell, tissue, and organ culture is a set of techniques designed for the growth and multiplication of cells and tissues using nutrient solutions in an aseptic and controlled environment. This technology explores conditions that promote cell division and genetic reprogramming in in vitro conditions.