AP BIOLOGY

THE CELL

CELL THEORY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
What does the term resolution refer to?
A
how many lenses a microscope uses
B
how much light is needed to see an image
C
how well structures close together can be distinguished
Explanation: 

Detailed explanation-1: -In microscopy, the term “resolution” is used to describe the ability of a microscope to distinguish details of a specimen or sample. In other words, the minimum distance between 2 distinct points of a specimen where they can still be seen by the observer or microscope camera as separate entities.

Detailed explanation-2: -Resolution is the ability to see two structures as two separate structures rather than as one fuzzy dot. A good example of this is when you look at the Moon with your naked eyes – you see a bright spot with patterns on it.

Detailed explanation-3: -Scientists think of resolution as the ability to tell that two objects that are very close together are distinct objects rather than just one.

Detailed explanation-4: -There are two quantities that encapsulate a pretty good idea of a microscope’s performance; they are the resolution and the field of view. Put simply, the resolution is the smallest feature you can see with the microscope, and the field of view is the size of the region of the sample you can see at once.

Detailed explanation-5: -Light microscopy is limited to a minimal resolution of approximately 200 nanometers (nm). The minimal resolution is defined as the distance between two points that are still distinguishable as two separate entities. The resolution is limited by physical properties of light and the lens of the microscope.

There is 1 question to complete.