AP BIOLOGY

Detailed explanation-1: -The higher the power of magnification, the smaller the field of view. For example, if you determine that your field of view is 2.5 mm in diameter using a 10X ocular and 4X objective, you will be able to determine what the field of view will be with the high power objective by using the above formula.

Detailed explanation-2: -Each time you switch to a higher power, the depth of focus is reduced. Therefore a smaller part of the specimen is in focus at higher power. The amount of light transmitted to your eye is greatest at the low power.

Detailed explanation-3: -The total magnification of a specimen is obtained by multiplying the magnification of the objective lens by the ocular lens. What you observe through the microscope is your field of view. As one increases the magnification, your field of view decreases. At low magnification you might see an entire organism.

Detailed explanation-4: -If we try to magnify further, we won’t be able to see any more detail than this – just like the digital photo above, the microscope will have reached the limit of its resolution. Different types of microscope have different resolving powers. Light microscopes let us distinguish objects as small as a bacterium.