AP BIOLOGY

Detailed explanation-1: -Many restriction enzymes make staggered cuts at or near their recognition sites, producing ends with a single-stranded overhang. If two DNA molecules have matching ends, they can be joined by the enzyme DNA ligase .

Detailed explanation-2: -Recombinant DNA Technology If genomic DNA is isolated from one organism and cut with one particular restriction enzyme, a specific set of fragments can be separated and identified by electrophoresis. If DNA from a different organism is cut by the same restriction enzyme, a different set of fragments will be generated.

Detailed explanation-3: -Using two different restriction enzyme sites can help ensure the correct orientation of the gene of interest when it is inserted and prevent the plasmid vector from ligating with itself.

Detailed explanation-4: -Recombinant DNA is the method of joining two or more DNA molecules to create a hybrid. The technology is made possible by two types of enzymes, restriction endonucleases and ligase.

Detailed explanation-5: -It’s important that both the desired section of DNA and the bacterial plasmid are cut using the same restriction enzymes to leave complementary sticky ends.