THE MOLECULAR BASIS OF INHERITANCE
CLONING GENES
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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True
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False
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Detailed explanation-1: -PCR requires thermal cycling for heating the DNA for strand separation and cooling it for replication.
Detailed explanation-2: -PCR cloning is a rapid method for cloning genes, and is often used for projects that require higher throughput than traditional cloning methods can accommodate. It allows for the cloning of DNA fragments that are not available in large amounts.
Detailed explanation-3: -Thermocyclers, or thermal cyclers, are instruments used to amplify DNA and RNA samples by the polymerase chain reaction. The thermocycler raises and lowers the temperature of the samples in a holding block in discrete, pre-programmed steps, allowing for denaturation and reannealing of samples with various reagents.
Detailed explanation-4: -PCR (Polymerase Chain Reaction) In this reaction, multiple copies of the gene (or DNA) of interest is synthesized in vitro using two sets of primers (small chemically synthesized oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. Hence, Statement 1 is correct.