THE MOLECULAR BASIS OF INHERITANCE
CLONING GENES
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Each cycle of the thermocycler doubles the number of DNA copies. How many cycles of the thermocycler are needed to make 16 copies of DNA?
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2
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3
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4
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5
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6
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Explanation:
Detailed explanation-1: -Each cycle doubles the copy number of the amplified gene: ten cycles ideally produces 2 4 8 16 32 64 128 256 512 1, 024 (210) copies. Thus, 30 cycles yields a (210x3) = 109-fold amplification. This produces a sufficient quantity of the gene region of interest for direct analysis, for example by DNA sequencing.
Detailed explanation-2: -The PCR process can amplify a single DNA to 2n times, where n is the number of cycles. Thus for 4 cycles of PCR, a given DNA template can be amplified to 16 duplicate strands.
Detailed explanation-3: -The number of double stranded DNA pieces is doubled in each cycle, so that after n cycles you have
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