THE MOLECULAR BASIS OF INHERITANCE
CLONING GENES
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Only PCR uses deoxynucleotides.
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Cycle Sequencing generates DNA products of different lengths.
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Only PCR analyzes DNA products using gel electrophoresis.
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Only Cycle Sequencing uses dideoxynucleotides.
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Detailed explanation-1: -Sanger sequencing differs from PCR in that only a single primer is used in the reaction. Typically, for a given PCR fragment, two Sanger sequencing reactions are set up, one for sequencing the forward strand, the other one for sequencing the reverse strand.
Detailed explanation-2: -The dideoxynucleotides, or ddNTPSs, differ from the deoxynucleotides by the lack of a free 3′ OH group on the five-carbon sugar. If a ddNTP is added to a growing a DNA strand, the chain is not extended any further because the free 3′ OH group needed to add another nucleotide is not available.
Detailed explanation-3: -Pyrosequencing is a method of DNA sequencing that differs from Sanger sequencing, in that it relies on the detection of pyrophosphate release and the generation of light on nucleotide incorporation, rather than chain termination with dideoxynucleotides.
Detailed explanation-4: -PCR is a one of the most common methods for obtaining targeted template for Sanger sequencing. By designing target-specific primers you can selectively amplify the target region to obtain sufficient template for sequencing. Successful PCR requires good quality input DNA and good primer design.