AP BIOLOGY

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THE MOLECULAR BASIS OF INHERITANCE

CLONING GENES

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
The alternative selectable marker ____ and chromogenic substrate ____ that selects recombinants on the basis of their ability to produce colour are
A
LacZ gene, X-gal
B
C
Amp R gene and X-gal
D
Explanation: 

Detailed explanation-1: -A scientist used a plasmid that contained ampicillin resistance gene and lacZ gene as selectable markers. He inserted the gene of interest within the ampicillin resistance gene instead of lacZ gene due to human error. The competent cells were allowed to take up the plasmid.

Detailed explanation-2: -So, the correct answer is ‘Galactosidase’.

Detailed explanation-3: -Direct visual selection method for the selection of recombinant host cells based on their inability to produce color in the presence of chromogenic substrate can be applied if the vector used is a Modified plasmid vector of E. coli is used. Thus, the answer is option A: Modified plasmid vector of E. coli.

Detailed explanation-4: -A person obtains transformants by inserting the desired gene within the coding sequence of enzyme -galactosidase.

There is 1 question to complete.
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