THE MOLECULAR BASIS OF INHERITANCE
CLONING GENES
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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DNA polymerase
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RNA polymerase
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ligase
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helicase
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Detailed explanation-1: -DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA.
Detailed explanation-2: -To repair these gaps, an enzyme called DNA ligase can be used to join the sugar–phosphate backbones.
Detailed explanation-3: -DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.
Detailed explanation-4: -In biochemistry, a ligase is an enzyme that can catalyze the joining (ligation) of two large molecules by forming a new chemical bond.
Detailed explanation-5: -Ligase, an enzyme that uses ATP to form bonds, is used in recombinant DNA cloning to join restriction endonuclease fragments that have annealed. The ligase commonly used is T4 DNA ligase, which was first isolated from E.