THE MOLECULAR BASIS OF INHERITANCE
CLONING GENES
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Clones with a vector antibiotic resistant
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DNA ligase is added to seal them together by forming phosphodiester bond
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Recognition sequence have cohesive end
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restriction enzyme makes single cut within the lacZ gene and many cuts in DNA
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Detailed explanation-1: -Restriction enzymes cleave DNA at specific nucleotide sequences. Restriction endonucleases cleave double-stranded DNA. These enzymes are sequence specific, and each enzyme acts at a limited number of sites in DNA called recognition, or cutting, sites.
Detailed explanation-2: -restriction enzyme, also called restriction endonuclease, a protein produced by bacteria that cleaves DNA at specific sites along the molecule. In the bacterial cell, restriction enzymes cleave foreign DNA, thus eliminating infecting organisms.
Detailed explanation-3: -Restriction enzymes cut DNA bonds between 3′ OH of one nucleotide and 5′ phosphate of the next one at the specific restriction site. Adding methyl groups to certain bases at the recognition sites on the bacterial DNA blocks the restriction enzyme to bind and protects the bacterial DNA from being cut by themselves.
Detailed explanation-4: -A restriction enzyme is a DNA-cutting enzyme that recognizes specific sites in DNA. Many restriction enzymes make staggered cuts at or near their recognition sites, producing ends with a single-stranded overhang.