THE MOLECULAR BASIS OF INHERITANCE
CLONING GENES
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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they are the building blocks used to build the copies of DNA
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they are the template that is used to make copies of the DNA
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they are the machine that changes temperature to make DNA copies
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they attach to either end of the DNA that needs to be copied
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Detailed explanation-1: -DNA Polymerase synthesises new strands of DNA complementary to the template DNA. The DNA polymerase can add a nucleotide to the pre-existing 3’-OH group only. Therefore, a primer is required. Thus, more nucleotides are added to the 3’ prime end of the DNA polymerase.
Detailed explanation-2: -PCR (Polymerase Chain Reaction) Because DNA polymerase can add a nucleotide only onto a preexisting 3’-OH group, it needs a primer to which it can add the first nucleotide. This requirement makes it possible to delineate a specific region of template sequence that the researcher wants to amplify.
Detailed explanation-3: -The various components required for PCR include a DNA sample, DNA primers, free nucleotides called ddNTPs, and DNA polymerase. The various components required for PCR include a DNA sample, DNA primers, free nucleotides called ddNTPs, and DNA polymerase.
Detailed explanation-4: -DNA polymerase is an essential component for PCR due to its key role in synthesizing new DNA strands.