THE MOLECULAR BASIS OF INHERITANCE
CLONING GENES
|
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
|
|
|
they are what makes copy of the DNA
|
|
|
they are used to build more copies of the DNA
|
|
|
they attach to sites on the DNA on either side of the DNA to be copied
|
|
|
they are what change temperature to copy the DNA
|
Detailed explanation-1: -The primers are designed to be complementary? in sequence to short sections of DNA on each end of the sequence to be copied. Primers serve as the starting point for DNA synthesis. The polymerase enzyme can only add DNA bases to a double strand of DNA.
Detailed explanation-2: -In the PCR method, a pair of primers hybridizes with the sample DNA and defines the region that will be amplified, resulting in millions and millions of copies in a very short timeframe. Primers are also used in DNA sequencing and other experimental processes.
Detailed explanation-3: -Why is it necessary to have a primer on each side of the DNA segment to be amplified? The primer is able to mark the spot where Taq polymerase must make matching strands.
Detailed explanation-4: -The synthesis of a primer is necessary because the enzymes that synthesize DNA, which are called DNA polymerases, can only attach new DNA nucleotides to an existing strand of nucleotides. The primer therefore serves to prime and lay a foundation for DNA synthesis.
Detailed explanation-5: -What is the function of the primers in PCR? They polymerize free nucleotides to form the new DNA strands. They are the monomer building blocks from which the DNA strand is synthesized.