AP BIOLOGY

THE MOLECULAR BASIS OF INHERITANCE

CLONING GENES

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Why is DNA polymerase needed in the PCR process?
A
they are the building blocks to make more DNA
B
it is used as a template to make more DNA
C
it attaches to either end of the gene to be copied
D
it is what makes a complementary copy of the template DNA strand
Explanation: 

Detailed explanation-1: -DNA polymerase is an essential component for PCR due to its key role in synthesizing new DNA strands.

Detailed explanation-2: -DNA template-the sample DNA that contains the target sequence. At the beginning of the reaction, high temperature is applied to the original double-stranded DNA molecule to separate the strands from each other.

Detailed explanation-3: -To amplify a segment of DNA using PCR, the sample is first heated so the DNA denatures, or separates into two pieces of single-stranded DNA. Next, an enzyme called “Taq polymerase” synthesizes-builds-two new strands of DNA, using the original strands as templates.

Detailed explanation-4: -Copying DNA in a test tube using PCR follows the principles of DNA replication inside living cells. DNA replication relies on the fact that the bases on both strands of DNA are complementary to each other.

There is 1 question to complete.