THE MOLECULAR BASIS OF INHERITANCE
CLONING GENES
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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it allows the primers to attach the DNA
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it allows the DNA polymerase to attach to the DNA to begin copying it
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it attaches the nucleotides to make DNA
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it unwinds the double stranded DNA so both strands can be used as a template to make more DNA
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Detailed explanation-1: -The high temperature causes the hydrogen bonds? between the bases in two strands of template DNA to break and the two strands to separate. This results in two single strands of DNA, which will act as templates for the production of the new strands of DNA.
Detailed explanation-2: -In order for PCR to be effective, single strands of DNA must be present to bind with their complementary primer strands. Because of this, the very first step in PCR is to denature the DNA. This step is carried out using high heat, usually around 95 degrees Celsius.
Detailed explanation-3: -replicated, so the first step of PCR is to denature the template DNA by heating the reaction to 94oC.-The high temperature causes the DNA double helix to separate by breaking the hydrogen bonds between the base pairs, resulting in a single-stranded DNA.
Detailed explanation-4: -To amplify a segment of DNA using PCR, the sample is first heated so the DNA denatures, or separates into two pieces of single-stranded DNA. Next, an enzyme called “Taq polymerase” synthesizes-builds-two new strands of DNA, using the original strands as templates.