THE MOLECULAR BASIS OF INHERITANCE
DNA MAKES RNA MAKES PROTEIN
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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True
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False
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Detailed explanation-1: -In the laboratory, restriction enzymes (or restriction endonucleases) are used to cut DNA into smaller fragments. The cuts are always made at specific nucleotide sequences. Different restriction enzymes recognise and cut different DNA sequences.
Detailed explanation-2: -Many restriction enzymes make staggered cuts at or near their recognition sites, producing ends with a single-stranded overhang. If two DNA molecules have matching ends, they can be joined by the enzyme DNA ligase. DNA ligase seals the gap between the molecules, forming a single piece of DNA.
Detailed explanation-3: -Definition. A restriction enzyme is a protein isolated from bacteria that cleaves DNA sequences at sequence-specific sites, producing DNA fragments with a known sequence at each end. The use of restriction enzymes is critical to certain laboratory methods, including recombinant DNA technology and genetic engineering.
Detailed explanation-4: -When foreign DNA and plasmid are both cut with the same restriction enzyme and mixed together, all molecules will form recombinants.