THE MOLECULAR BASIS OF INHERITANCE
DNA MAKES RNA MAKES PROTEIN
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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False
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True
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Detailed explanation-1: -Ligases join fragments of DNA together by catalysing the formation of bonds between neighbouring nucleotides.
Detailed explanation-2: -DNA ligases catalyse the formation of a phosphodiester bond between adjacent 3′ hydroxyl and 5′ phosphate terminal groups in double stranded DNA.
Detailed explanation-3: -DNA ligase is used in both DNA repair and DNA replication (see Mammalian ligases). In addition, DNA ligase has extensive use in molecular biology laboratories for recombinant DNA experiments (see Research applications). Purified DNA ligase is used in gene cloning to join DNA molecules together to form recombinant DNA.
Detailed explanation-4: -DNA ligase works by catalyzing the formation of a phosphodiester bond between nucleotides on one strand of a double stranded DNA molecule. DNA ligase is able to create a covalent linkage between the 5’ phosphate group of one chain with the adjacent 3’-OH group of another.