THE MOLECULAR BASIS OF INHERITANCE
DNA MAKES RNA MAKES PROTEIN
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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First, cut the gene and plasmid with the same restriction enzyme. Second, insert the gene into the plasmid.
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First, insert the gene into the plasmid. Second, cut the gene and plasmid with the same restriction enzyme.
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First, cut the gene and plasmid with different restriction enzymes. Second, insert the gene into the plasmid.
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First, insert the gene into the plasmid. Second, cut the gene and plasmid with different restriction enzymes.
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Detailed explanation-1: -The restriction enzymes cuts at fixed sequences, therefore same restriction enzyme must be used since it forms fragments with complementary sticky ends, therefore facilitating the formation of bonds between them.
Detailed explanation-2: -So, if the donor DNA and the vector DNA are both cut with the same enzyme, there is a strong possibility that the donor fragments and the cut vector will splice together because of the complementary overhangs. The resulting molecule is called recombinant DNA.
Detailed explanation-3: -Many restriction enzymes make staggered cuts at or near their recognition sites, producing ends with a single-stranded overhang. If two DNA molecules have matching ends, they can be joined by the enzyme DNA ligase. DNA ligase seals the gap between the molecules, forming a single piece of DNA.
Detailed explanation-4: -(1) Isolate the DNA sequence gene of interest. (2) Insert your DNA of interest into the vector plasmid: (a) cut both vector DNA and gene of interest with the same restriction enzymes, (b) mix the DNAs: they join by base pairing, (c) add DNA ligase to bond the pieces covalently. The result is a recombinant plasmid.