THE MOLECULAR BASIS OF INHERITANCE
DNA MAKES RNA MAKES PROTEIN
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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plasmid DNA
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DNA containing insulin gene
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restriction enzymes
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Detailed explanation-1: -The Genetics of the Insulin Gene This is encoded by a 14kb sequence on the INS gene. In most animals including humans, a single gene for insulin is found. The human gene is located on the short arm of chromosome 11 at position 15.5 (11p15. 5).
Detailed explanation-2: -insert the human insulin gene into the plasmid. Researchers return the plasmid to the bacteria and… put the “recombinant” bacteria in large fermentation tanks. There, the recombinant bacteria use the gene to begin producing human insulin.
Detailed explanation-3: -The human insulin gene is extracted from human cells using restriction enzymes. This is inserted into a plasmid using an enzyme called ligase, producing recombinant DNA. The recombinant plasmid is placed inside a bacterium which replicates (the bacterium is now transgenic because it contains DNA from another species).
Detailed explanation-4: -Recombinant human insulin has been produced predominantly using E. coli and Saccharomyces cerevisiae for therapeutic use in human.