AP BIOLOGY

THE MOLECULAR BASIS OF INHERITANCE

DNA MAKES RNA MAKES PROTEIN

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Scientists can transform plant cells without using plamids by
A
injecting DNA into the plant cells.
B
removing the plant cell walls and then mixing the cells with DNA.
C
Both A and B.
D
None of the above.
Explanation: 

Detailed explanation-1: -For GM plants, the bacterium most frequently used is called Agrobacterium tumefaciens. The gene of interest is transferred into the bacterium and the bacterial cells then transfer the new DNA to the genome of the plant cells. The plant cells that have successfully taken up the DNA are then grown to create a new plant.

Detailed explanation-2: -The molecular basis of genetic transformation of plant cells by Agrobacterium is transfer from the bacterium and integration into the plant nuclear genome of a region of a large tumor-inducing (Ti) or rhizogenic (Ri) plasmid resident in Agrobacterium (Fig.

Detailed explanation-3: -Using Agrobacterium for plant transformation. Agrobacterium-mediated transformation is the most commonly used method for plant genetic engineering (Bartlett et al., 2008; Leplé et al., 1992; May et al., 1995; Sun et al., 2006; Tsai et al., 1994; Tzfira et al., 1997).

Detailed explanation-4: -The most commonly used plant transformation vectors are termed binary vectors because of their ability to replicate in both E. coli, a common lab bacterium and Agrobacterium tumefaciens, a bacterium used to insert the recombinant (customized) DNA into plants.

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