THE MOLECULAR BASIS OF INHERITANCE
DNA MAKES RNA MAKES PROTEIN
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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sticky ends
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DNA fragments
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recombinant DNA
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Detailed explanation-1: -These kinds of ends are called sticky or cohesive ends because base pairing between them can stick the DNA molecule back together again. Hence, cohesive ends of DNA are produced by restriction endonuclease when enzymatic digestion of DNA strands leave unpaired bases on both ends of the two strands.
Detailed explanation-2: -EcoRI is a restriction enzyme that is derived from the bacteria E. coli. This restriction enzyme produces sticky ends when it cuts DNA at the restriction site. EcoRI recognizes a specific sequence on DNA: GAATTC.
Detailed explanation-3: -The sticky ends, a.k.a. cohesive ends, have unpaired DNA nucleotides on either 5’-or 3’-strand, which are known as overhangs. These overhangs are most often generated by a staggered cut of restriction enzymes.
Detailed explanation-4: -These are called sticky ends because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase.
Detailed explanation-5: -Restriction enzymes are DNA-cutting enzymes.