THE MOLECULAR BASIS OF INHERITANCE
DNA MAKES RNA MAKES PROTEIN
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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plasmid
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restriction enzyme
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ligase
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protein expression
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Detailed explanation-1: -Ligase is the enzyme that seals the gaps in the DNA strand created by restriction endonucleases.
Detailed explanation-2: -Restriction enzymes are also called “molecular scissors” as they cleave DNA at or near specific recognition sequences known as restriction sites. These enzymes make one incision on each of the two strands of DNA and are also called restriction endonucleases.
Detailed explanation-3: -SmaI is an example of a restriction enzyme that cuts straight through the DNA strands, creating DNA fragments with a flat or blunt end. Other restriction enzymes, like EcoRI, cut through the DNA strands at nucleotides that are not exactly opposite each other.
Detailed explanation-4: -The enzyme Restriction endonuclease is used for this purpose. This enzyme is known as ‘genetic scissors’. Q.
Detailed explanation-5: -A restriction enzyme is a protein isolated from bacteria that cleaves DNA sequences at sequence-specific sites, producing DNA fragments with a known sequence at each end. The use of restriction enzymes is critical to certain laboratory methods, including recombinant DNA technology and genetic engineering.