THE MOLECULAR BASIS OF INHERITANCE
DNA MAKES RNA MAKES PROTEIN
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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plasmid
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restriction enzyme
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ligase
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protein expression
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Detailed explanation-1: -Ligase, an enzyme that uses ATP to form bonds, is used in recombinant DNA cloning to join restriction endonuclease fragments that have annealed. The ligase commonly used is T4 DNA ligase, which was first isolated from E. coli that were infected with the lytic bacteriophage T4.
Detailed explanation-2: -DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA.
Detailed explanation-3: -DNA ligase is a basic class of enzymes necessary in joining the strands of DNA together by catalyzing the association of the phosphodiester bond.
Detailed explanation-4: -Ligase is very useful during DNA replication due to the formation of Okazaki fragments, which needs ligation after the process of replication is over.
Detailed explanation-5: -DNA ligase is an enzyme that can join two DNA molecules. You can think of DNA ligase as molecular glue. DNA ligase joins two DNA molecules together by forming a phosphodiester bond between the two molecules.