AP BIOLOGY

Detailed explanation-1: -Because DNA polymerase can only synthesize DNA in a 5′ to 3′ direction, the other new strand is put together in short pieces called Okazaki fragments.

Detailed explanation-2: -DNA Polymerase Only Moves in One Direction After a primer is synthesized on a strand of DNA and the DNA strands unwind, synthesis and elongation can proceed in only one direction. As previously mentioned, DNA polymerase can only add to the 3’ end, so the 5’ end of the primer remains unaltered.

Detailed explanation-3: -DNA polymerases can only make DNA in the 5’ to 3’ direction, and this poses a problem during replication. A DNA double helix is always anti-parallel; in other words, one strand runs in the 5’ to 3’ direction, while the other runs in the 3’ to 5’ direction.

Detailed explanation-4: -coli polymerase I has 3′ to 5′ as well as 5′ to 3′ exonuclease activity. The 5′ to 3′ exonuclease operates in the direction of DNA synthesis and helps remove RNA primers from Okazaki fragments.