AP BIOLOGY

Detailed explanation-1: -If two DNA molecules have matching ends, they can be joined by the enzyme DNA ligase . DNA ligase seals the gap between the molecules, forming a single piece of DNA.

Detailed explanation-2: -Okazaki fragments are short sequences of DNA nucleotides (approximately 150 to 200 base pairs long in eukaryotes) which are synthesized discontinuously and later linked together by the enzyme DNA ligase to create the lagging strand during DNA replication.

Detailed explanation-3: -The DNA ligase enzyme family catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA. This activity can seal nicks in duplex DNA or join double-stranded DNA fragments having either blunt or cohesive ends.

Detailed explanation-4: -DNA ligase is involved in replication of DNA during cell division. The enzyme’s ability to attach two DNA strands makes it a valuable tool in biotechnology.