AP BIOLOGY

Detailed explanation-1: -DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA. In DNA cloning, restriction enzymes and DNA ligase are used to insert genes and other pieces of DNA into plasmids.

Detailed explanation-2: -DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.

Detailed explanation-3: -DNA ligases play an essential role in maintaining genomic integrity by joining breaks in the phosphodiester backbone of DNA that occur during replication and recombination, and as a consequence of DNA damage and its repair.

Detailed explanation-4: -Ligases join fragments of DNA together by catalysing the formation of bonds between neighbouring nucleotides.

Detailed explanation-5: -DNA ligase binds the Okazaki fragments together. In the four stage of DNA replication, the enzyme DNA ligase binds the Okazaki fragments together to make a continuous daughter strand.