AP BIOLOGY

THE MOLECULAR BASIS OF INHERITANCE

DNA REPLICATION

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
The polymerase chain reaction (PCR) is used to
A
insert DNA from one organism into a new host.
B
screen for a particular gene.
C
produce many copies of a selected DNA sequence.
Explanation: 

Detailed explanation-1: -Polymerase chain reaction, or PCR, is a technique to make many copies of a specific DNA region in vitro (in a test tube rather than an organism). PCR relies on a thermostable DNA polymerase, Taq polymerase, and requires DNA primers designed specifically for the DNA region of interest.

Detailed explanation-2: -The cycle of denaturing and synthesizing new DNA is repeated as many as 30 or 40 times, leading to more than one billion exact copies of the original DNA segment. The entire cycling process of PCR is automated and can be completed in just a few hours.

Detailed explanation-3: -PCR is a common tool used in medical and biological research labs. It is used in the early stages of processing DNA for sequencing?, for detecting the presence or absence of a gene to help identify pathogens? during infection, and when generating forensic DNA profiles from tiny samples of DNA.

Detailed explanation-4: -Polymerase Chain Reaction (PCR) PCR involves using short synthetic DNA fragments called primers to select a segment of the genome to be amplified, and then multiple rounds of DNA synthesis to amplify that segment.

Detailed explanation-5: -Polymerase chain reaction (PCR) Polymerase chain reaction (PCR) enables researchers to produce millions of copies of a specific DNA sequence in approximately two hours. This automated process bypasses the need to use bacteria for amplifying DNA.

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