THE MOLECULAR BASIS OF INHERITANCE
DNA REPLICATION
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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attach free nucleotides to the growing chain
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remove bases that might have been inserted correctly.
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cut the two strands of the DNA molecule prior to replication.
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seal new short stretches of nucleotides into one continuous strand.
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Detailed explanation-1: -DNA ligase joins the Okazaki fragments together into a single DNA molecule. Helicase opens up the DNA at the replication fork. Single-strand binding proteins coat the DNA around the replication fork to prevent rewinding of the DNA. Topoisomerase works at the region ahead of the replication fork to prevent supercoiling.
Detailed explanation-2: -DNA ligases play an essential role in maintaining genomic integrity by joining breaks in the phosphodiester backbone of DNA that occur during replication and recombination, and as a consequence of DNA damage and its repair.
Detailed explanation-3: -Posted July 22, 2020. No. The role of DNA ligase in DNA replication is to join the Okazaki fragments synthesized on the lagging strand into a continuous strand. In the case of leading strand, the nucleotides are added to the growing 3’ end continuously.
Detailed explanation-4: -The ligase joins the two fragments of DNA to form a longer strand of DNA by “pasting” them together.
Detailed explanation-5: -DNA ligases seal 5′-PO4 and 3′-OH polynucleotide ends via three nucleotidyl transfer steps involving ligase-adenylate and DNA-adenylate intermediates.