AP BIOLOGY

Detailed explanation-1: -RNA primers are removed and replaced with DNA by DNA polymerase I. The gaps between DNA fragments are sealed by DNA ligase.

Detailed explanation-2: -DNA polymerase I excises the RNA primers and replaces them with the appropriate deoxyribonucleotides. DNA ligase joins the gaps in the Okazaki fragments by the creation of a phosphodiester bond.

Detailed explanation-3: -As synthesis proceeds, an enzyme removes the RNA primer, which is then replaced with DNA nucleotides, and the gaps between fragments are sealed by an enzyme called DNA ligase.

Detailed explanation-4: -Primase is the enzyme that synthesizes RNA primers. Primers are oligonucleotides that are complementarily bound to a DNA template and from which DNA polymerases elongate. Special proteins are responsible for loading primase at the origin of replication so that leading strand DNA synthesis can commence.