THE MOLECULAR BASIS OF INHERITANCE
DNA REPLICATION
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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What does DNA ligase do?
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break hydrogen bonds between bases
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add new nucleotides
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fill gaps between nucleotides (for example, at Okazaki fragments)
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prevent the double helix from re-forming hydrogen bonds
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Explanation:
Detailed explanation-1: -DNA ligases are best known for their role in joining adjacent Okazaki fragments at the lagging strand of the replication fork; however, they are essentially involved in any process that requires sealing of phosphodiester bonds from the DNA backbone.
Detailed explanation-2: -DNA polymerase I is recognized as an enzyme that fills any remaining gaps between Okazaki fragments and removes RNA primers with a duplex-specific 5′-3′ exonuclease activity, providing a nick that can be sealed by DNA ligase 31, 32.
Detailed explanation-3: -During lagging strand synthesis, DNA Ligase 1 (Lig1) cooperates with the sliding clamp PCNA to seal the nicks between Okazaki fragments generated by Pol
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