THE MOLECULAR BASIS OF INHERITANCE
DNA REPLICATION
|
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
|
|
|
add nucleotides after helicase unwinds the double helix
|
|
|
replace RNA primer with DNA
|
|
|
relieve strain ahead of the replication fork
|
|
|
protect the strands of DNA from re-forming hydrogen bases
|
Detailed explanation-1: -Removal of RNA primers and joining of Okazaki fragments. Because of its 5′ to 3′ exonuclease activity, DNA polymerase I removes RNA primers and fills the gaps between Okazaki fragments with DNA.
Detailed explanation-2: -RNA primers are removed and replaced with DNA by DNA polymerase I. The gaps between DNA fragments are sealed by DNA ligase.
Detailed explanation-3: -The physiological function of Pol I is mainly to support repair of damaged DNA, but it also contributes to connecting Okazaki fragments by deleting RNA primers and replacing the ribonucleotides with DNA.
Detailed explanation-4: -DNA polymerase I (pol I) processes RNA primers during lagging-strand synthesis and fills small gaps during DNA repair reactions.
Detailed explanation-5: -DNA Polymerase I, Bacterial DNA polymerase I of eubacteria functions in vivo to synthesize short stretches of DNA during excision repair and to remove RNA primers and fill the gaps between Okazaki fragments in lagging strand replication.