THE MOLECULAR BASIS OF INHERITANCE
DNA REPLICATION
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Unwind the double stranded DNA
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Scan for mismatches
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Add primers to the lagging strand
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Fix breaks in the sugar-phosphate backbone
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Detailed explanation-1: -DNA helicases catalyze the disruption of the hydrogen bonds that hold the two strands of double-stranded DNA together. This energy-requiring unwinding reaction results in the formation of the single-stranded DNA required as a template or reaction intermediate in DNA replication, repair and recombination.
Detailed explanation-2: -DNA helicases are a class of molecular motors that catalyze processive unwinding of double stranded DNA.
Detailed explanation-3: -Helicases are enzymes that use ATP-driven motor force to unwind double-stranded DNA or RNA. Recently, increasing evidence demonstrates that some helicases also possess rewinding activity-in other words, they can anneal two complementary single-stranded nucleic acids.
Detailed explanation-4: -In this paper, we study the mechanism of DNA unwinding by bacteriophage T7 helicase that is involved in DNA replication. During replication, the helicase has to unwind a long stretch of DNA, and that requires the helicase to couple strand-separation activity to translocation.
Detailed explanation-5: -coli, the DnaB Helicase (Figure 9.5) is responsible for unwinding the two parent DNA strands to unwind and separating them from one another to form a “Y”-shaped replication fork.