THE MOLECULAR BASIS OF INHERITANCE
DNA REPLICATION
|
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
|
|
|
To add appropriate nucleotides in the 3’
|
|
|
To remove the RNA primers and replace them with DNA
|
|
|
To join together the Okazaki fragments
|
|
|
To join together both strands of DNA to the histones
|
Detailed explanation-1: -DNA polymerase I of eubacteria functions in vivo to synthesize short stretches of DNA during excision repair and to remove RNA primers and fill the gaps between Okazaki fragments in lagging strand replication.
Detailed explanation-2: -Primase synthesizes RNA primers complementary to the DNA strand. DNA polymerase III extends the primers, adding on to the 3’ end, to make the bulk of the new DNA. RNA primers are removed and replaced with DNA by DNA polymerase I.
Detailed explanation-3: -Because of its 5′ to 3′ exonuclease activity, DNA polymerase I removes RNA primers and fills the gaps between Okazaki fragments with DNA.
Detailed explanation-4: -The physiological function of Pol I is mainly to support repair of damaged DNA, but it also contributes to connecting Okazaki fragments by deleting RNA primers and replacing the ribonucleotides with DNA.
Detailed explanation-5: -RNA Polymerase (Pol) I produces ribosomal (r)RNA, an essential component of the cellular protein synthetic machinery that drives cell growth, underlying many fundamental cellular processes.