AP BIOLOGY

Detailed explanation-1: -In 1958, Matthew Meselson and Franklin Stahl conducted an experiment on E.coli which divides in 20 minutes, to study the replication of DNA.

Detailed explanation-2: -The experiment done by Meselson and Stahl demonstrated that DNA replicated semi-conservatively, meaning that each strand in a DNA molecule serves as a template for synthesis of a new, complementary strand. Although Meselson and Stahl did their experiments in the bacterium E.

Detailed explanation-3: -Meselson & Stahl reasoned that these experiments showed that DNA replication was semi-conservative: the DNA strands separate and each makes a copy of itself, so that each daughter molecule comprises one “old” and one “new” strand.

Detailed explanation-4: -Meselson and Stahl conducted an experiment to demonstrate the semi-conservative nature of DNA replication. They used two cultures of E. coli, one growing in medium with N14 isotope while other growing in medium with N15 isotopes. They were allowed to grow separately for several generations.

Detailed explanation-5: -Solution : Process used by Meselson and Stahl for studying semiconservative replication of DNA was CsCl density gradient centrifugation.