AP BIOLOGY

THE MOLECULAR BASIS OF INHERITANCE

DNA REPLICATION

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
What prevents DNA strands from reannealing at the replication fork?
A
Helicase
B
Topoisomerase
C
Ligase
D
Single-stranded binding proteins
Explanation: 

Detailed explanation-1: -Because the two helical DNA strands are wound together, separation of the strands without unwinding the entire DNA molecule requires breaking of a strand via DNA helicase enzyme. Single-stranded binding protein then binds the unwound DNA, preventing re-annealing of the two parent strands.

Detailed explanation-2: -Single strand binding protein (ssb protein) binds to separated strands of DNA and prevents reannealing.

Detailed explanation-3: -Single-stranded DNA (ssDNA) binding protein plays an important role in the DNA replication process in a wide range of organisms. It binds to ssDNA to prevent premature reannealing and to protect it from degradation.

Detailed explanation-4: -During DNA replication, the single-stranded DNA binding protein (SSB) wraps single-stranded DNA (ssDNA) with high affinity to protect it from degradation and prevent secondary structure formation.

Detailed explanation-5: -Single-stranded DNA-binding protein (SSB) binds to single-stranded regions of DNA (ssDNA) to protect it from annealing, prevention of secondary structure formation and digestion.

There is 1 question to complete.