AP BIOLOGY

THE MOLECULAR BASIS OF INHERITANCE

DNA REPLICATION

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
What was the third solution that the was bacteria was placed in?
A
15N
B
14N
C
CsCl
D
ClCs
Explanation: 

Detailed explanation-1: -The cesium chloride acted as a buffer to stabilize and protect extracted DNA. B: The cesium chloride caused the DNA of early generations of bacterial cells to be heavier than the DNA of later generations of bacterial cells, thereby allowing Meselson and Stahl to determine that DNA replication is semiconservative.

Detailed explanation-2: -Since CsCl has a density almost equivalent to that of DNA, it helps in isolating DNA from a sample containing DNA, RNA, and proteins. Since proteins are heavier they sediment in the heaviest, bottom-most layer while RNA being lighter is present at the topmost layer with the lowest density.

Detailed explanation-3: -Conclusion. The experiment done by Meselson and Stahl demonstrated that DNA replicated semi-conservatively, meaning that each strand in a DNA molecule serves as a template for synthesis of a new, complementary strand.

Detailed explanation-4: -Caesium chloride is widely used medicine structure in isopycnic centrifugation for separating various types of DNA. It is a reagent in analytical chemistry, where it is used to identify ions by the color and morphology of the precipitate.

There is 1 question to complete.