THE MOLECULAR BASIS OF INHERITANCE
DNA REPLICATION
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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alpha
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beta
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delta
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epsilon
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gamma
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Detailed explanation-1: -Nucleotide excision-repair uses DNA polymerases delta or epsilon to resynthesize the bases removed during repair of pyrimidine dimers and other bulky adducts in DNA.
Detailed explanation-2: -In addition to its role in DNA replication, DNA polymerase epsilon fulfils roles in the DNA synthesis step of nucleotide excision and base excision repair, and has been implicated in recombinational processes in the cell.
Detailed explanation-3: -Either DNA polymerase epsilon or DNA polymerase delta along with DNA ligase can be used to repair UV-damaged DNA. However, it is found that DNA polymerase delta require the presence of both RFC and PCNA in order in DNA repair. In addition, it only produces small amount of fractionated DNA ligated products.
Detailed explanation-4: -DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3’-5’ exonuclease (PubMed:6340117).
Detailed explanation-5: -Thus, it appears that a functional DNA polymerase III is required for the optimal repair of DNA damage by MMS or hydrogen peroxide.