AP BIOLOGY

Detailed explanation-1: -On the lagging strand, DNA synthesis restarts many times as the helix unwinds, resulting in many short fragments called “Okazaki fragments.” DNA ligase joins the Okazaki fragments together into a single DNA molecule.

Detailed explanation-2: -DNA ligase I is responsible for joining Okazaki fragments together to form a continuous lagging strand.

Detailed explanation-3: -The Okazaki fragments must be attached together into one continuous strand after replication. This is accomplished by the enzyme DNA ligase, which seals the sugar phosphate backbone of the Okazaki fragments.

Detailed explanation-4: -Abstract. DNA ligase 1 (LIG1, Cdc9 in yeast) finalizes eukaryotic nuclear DNA replication by sealing Okazaki fragments using DNA end-joining reactions that strongly discriminate against incorrectly paired DNA substrates.