THE MOLECULAR BASIS OF INHERITANCE
DNA REPLICATION
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Topoisomerase II-can remove both positive and negative supercoil in the DNA duplex
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Polymerase I-larger fragment responsible for exonuclease activity
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DnaA protein-responsible for “melting” of the DNA double helix during replication
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DnaB protein-attaches to the newly unwounded single strand of DNA to prevent folding of the strand
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Detailed explanation-1: -Which of the following is correctly matched with its subsequent role? iii) DnaB protein-also known as helicase helps in unwinding of DNA duplex to form the open complex.
Detailed explanation-2: -Which of the following replication proteins is used to unwind the DNA double helix? Explanation: DNA helicase unwinds the double helix, separating the two strands so they may be replicated by DNA polymerase.
Detailed explanation-3: -Additional replication proteins are needed to help in opening the double helix and thus provide the appropriate single-stranded DNA template for the DNA polymerase to copy. Two types of protein contribute to this process-DNA helicases and single-strand DNA-binding proteins.
Detailed explanation-4: -Helicase and single-stranded DNA-binding proteins then act to continue unwinding and exposing the template DNA, and primase initiates the synthesis of leading strands.
Detailed explanation-5: -Each strand of DNA acts as a template for synthesis of a new, complementary strand. Replication produces two identical DNA double helices, each with one new and one old strand.