THE MOLECULAR BASIS OF INHERITANCE
DNA REPLICATION
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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DNA polymerase adds new nucleotides
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DNA polymerase adds new nucletoides
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Ligase seals the DNA fragments
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Primase adds primers
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Detailed explanation-1: -The DNA ligase enzyme family catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA. This activity can seal nicks in duplex DNA or join double-stranded DNA fragments having either blunt or cohesive ends.
Detailed explanation-2: -If two DNA molecules have matching ends, they can be joined by the enzyme DNA ligase. DNA ligase seals the gap between the molecules, forming a single piece of DNA.
Detailed explanation-3: -DNA ligases seal 5′-PO4 and 3′-OH polynucleotide ends via three nucleotidyl transfer steps involving ligase-adenylate and DNA-adenylate intermediates.
Detailed explanation-4: -Ligase can then connect two complementary sticky ends from the plasmid and genomic DNA to generate a new, double-stranded DNA sequence.