AP BIOLOGY

THE MOLECULAR BASIS OF INHERITANCE

RECOMBINANT DNA

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
A cloning vector is
A
An agent that transfers a pathogen.
B
An agent that transfers a foreign gene from one DNA molecule to another.
C
An agent that transfers a DNA segment to a RNA molecule.
D
An agent that transfers a RNA segment to a DNA molecule.
Explanation: 

Detailed explanation-1: -In general, cloning vectors are plasmids that are used primarily to propagate DNA. They replicate in E. coli to high copy numbers and contain a multiple cloning site (also called a polylinker) with restriction sites used for inserting a DNA fragment.

Detailed explanation-2: -Gene Transfer: The introduction of new DNA into an existing organism’s cell, usually by vectors such as plasmids and modified viruses.

Detailed explanation-3: -A DNA molecule that is incorporated into either a cloning vector or a chromosomal site. A DNA which is other than the host cell is called foreign DNA. There is no alien DNA. But Alien gene is termed as that a gene transferred to the cultivated species from another related or unrelated species.

Detailed explanation-4: -A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium.

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