THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Examining the cells with an electron microscope
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Using radioactive tracers to locate plasmids
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Exposing the bacteria to an antibiotic that kills the cells lacking the plasmids
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Exposing the bacteria to an antibiotic with X-gal containing medium
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Detailed explanation-1: -Cells containing recombinant plasmids can often be identified as containing recombinant plasmids by screening for the insertional inactivation of a second genetic marker on the plasmid.
Detailed explanation-2: -Once a recombinant plasmid is made that contains a gene of interest, such as insulin, the plasmid can enter bacterial cells by a process called transformation.
Detailed explanation-3: -Blue-white screening is a rapid and efficient technique for the identification of recombinant bacteria. It relies on the activity of -galactosidase, an enzyme occurring in E. coli, which cleaves lactose into glucose and galactose.
Detailed explanation-4: -The most accurate way to verify your recombinant colonies is by Sanger sequencing. Plasmid DNA is first isolated from an overnight bacterial culture. Once completed, the insert can be identified using sequencing primers appropriate for the selected vector.